PKM Antibody

SKU: orb420362

Description

PKM2 antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:50,000, WB: 1.0 ug/ml
• Reactivity: Human
• Application Notes:
  This affinity purified antibody has been tested for use in western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band 56-58 kDa in size corresponding to PKM2 by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Affinity purified Anti-PKM2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the internal portion of human PKM2 protein.
• Target: PKM
• Purity: Anti-PKM2 is directed against human PKM2 at an internal position. This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads. A BLAST analysis was used to suggest reactivity with this protein in African frog and rat based on 100% homology and rabbit and mouse based on 93.3% homology for the immunogen sequence.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-PKM2 antibody, PKM 2, PKM-2, Pyruvate kinase PKM, Cytosolic thyroid hormone-binding protein, CTHBP, Opa-interacting protein 3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, THBP1, Tumor M2-PK, p58, OIP3, OIP-3, PK2, PK3

Western Blot of Rabbit Anti-PKM2 antibody. Lane 1 and 2: iPSC lysate. Lane 3 and 4: myeloid lysate (iPSC derived). Load: 100 µg per lane. Primary antibody: PKM2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% Odyssey blocking buffer for 120 min at RT. Predicted/Observed size: 57 kda for PKM2. Other band(s): PKM2 splice variants and isoforms as PKM2 is a highly modified protein.

Quick Database Links

Gene Symbol

PKM

UniProt

P14618

RefSeq (Protein):NP_001193725.1