PJ-34 HCl

SKU: orb1306430

Description

PJ34 hydrochloride

Research Area

Neuroscience

Key Properties

• CAS Number: 344458-15-7
• MW: 331.81
• Purity: >98%
• Formula: C₁₇H₁₇N₃O₂
• SMILES: Cl.CN(C)CC(=O)Nc1ccc2[nH]c(=O)c3ccccc3c2c1
• Target: PARP
• Solubility: Soluble in DMSO (up to 30 mg/ml) or in Water (up to 20 mg/ml).

Bioactivity

• Target IC50:
  PARP:20 nM(EC50)|PARP1:110 nM|PARP2:86 nM
• In Vivo:
  In peroxynitrite-induced cells (EC 50=20 nM), PJ34 was able to inhibit the onset of cell necrosis.
• In Vitro:
  In peroxynitrite-induced cells (EC 50=20 nM), PJ34 was able to inhibit the onset of cell necrosis.
• Cell Research:
  PJ34 is dissolved in 100% DMSO at 10 mM and then diluted in DMEM without serum. PC12 cell cultured are grown in Dulbecco's modified Eagle's medium supplemented with 5% (v/v) fetal calf serum, 5% (v/v) horse serum, and a 1% (v/v) penicillin-streptomycin antibiotics mixture. Cells are grown in an atmosphere of 95% air and 5% CO2 at 37°C. For all experiment, cells are seeded at a density of 4×104 cells/well in 96-well culture plates and allowed to attach overnight. For assessment of cell viability, hydrogen peroxide-induced cytotoxicity is quantified by a standard measurement of LDH release with the use of the LDH assay kit. Briefly, 6 h after hydrogen peroxide exposure, 20 μL of medium of each well is collected, and the solution prepared from LDH assay kit is added. After incubation at room temperature for 30 min, the reaction is stopped by addition of 1 N HCl, and absorbance is measured at 450 nm using a microplate reader.

Storage & Handling

• Storage: RT
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PARP, inhibit, Inhibitor, poly ADP ribose polymerase, PJ 34, PJ 34 Hydrochloride, PJ34, PJ-34, PJ34 HCl, PJ34 hydrochloride, PJ34 Hydrochloride, PJ-34 Hydrochloride, PJ-34 hydrochloride

Compound Identifiers

PubChem CID

16760621