Pitstop 2

SKU: orb1218839

Description

Pitstop 2 is a inhibitor of clathrin, and inhibits clathrin-mediated endocytosis (CME) by associating with the terminal domain of clathrin. It has the potential for anti-cancer research.

Key Properties

• CAS Number: 1419320-73-2
• MW: 473.36
• Purity: >98% (HPLC)
• Formula: C₂₀H₁₃BrN₂O₃S₂
• SMILES: O=S(C1=C2C=CC=CC2=CC=C1)(NC(S/C3=C\C4=CC=C(Br)C=C4)=NC3=O)=O.[(Z)]
• Target: Apoptosis
• Solubility: DMSO : 62.5 mg/mL (132.03 mM; Need ultrasonic)

Bioactivity

• In Vitro:
  Pitstop 2 (20-40 μM; 30 min) inhibits the endocytosis of transferrin in J774A.1 macrophages and does not compromise cell viability. Pitstop 2 (20-40 μM; 30 min) does not affect the internalization of cholera toxin B in J774A.1 macrophages. Pitstop 2 (0.001-100 μM; 6 h) inhibits the mitotic spindle and impairs mitotic progression in HeLa cells. Pitstop 2 (1-30 μM; 24 h) induces apoptosis and inhibits cell growth in dividing cancer cells. Pitstop 2 (1-30 μM; 48 h) does not affect the growth and viability of non-tumourigenic NIH3T3 fibroblasts. Cell Viability Assay Cell line: HeLa cells. Concentration: 1, 3, 10, 30 μM. Incubation time: 24 hours. Result: Reduced the total number of viable HeLa cells markedly in a dose-dependent manner.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Apoptosis