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Catalog Number | orb1239874 |
---|---|
Category | Antibodies |
Description | PION Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | PION antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human PION.The immunogen is located within amino acids 770 - 820 of PION. |
Antibody Type | Primary Antibody |
Concentration | 1 mg/mL |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | PION |
UniProt ID | A4D1B5 |
NCBI | NP_059135 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PION Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | PION Antibody: PION, PION, Gamma-secretase-activat Read more... |
Note | For research use only |
Application notes | PION antibody can be used for detection of PION by Western blot at 0.25 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of PION in EL4 cell lysate with PION antibody at 0.25 µg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of PION in human brain tissue with PION antibody at 5 µg/mL.
Immunofluorescence of PION in Human Brain cells with PION antibody at 20 µg/mL.
Immunohistochemistry of PION in mouse brain tissue with PION antibody at 5 µg/mL.
Immunofluorescence of PION in mouse brain tissue with PION antibody at 20 µg/mL. Red: PION Antibody (orb1239874) Blue: DAPI staining.
WB | |
Bovine, Canine, Equine, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
Rat | |
0.32-20 ng/mL | |
0.116 ng/mL |
WB | |
Bovine, Canine, Equine, Human, Rabbit, Rat | |
Rabbit | |
Polyclonal | |
HRP |