PION Antibody

• Catalog Number: orb1239874
• Category: Antibodies
• Description: PION Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: PION antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human PION.The immunogen is located within amino acids 770 - 820 of PION.
• Concentration: 1 mg/mL
• Dilution range: PION antibody can be used for detection of PION by Western blot at 0.25 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PION
• UniProt ID: A4D1B5
• NCBI: NP_059135
• Storage: PION antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: PION Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: PION Antibody: PION, PION, Gamma-secretase-activat
• Note: For research use only
• Application notes: PION antibody can be used for detection of PION by Western blot at 0.25 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PION in EL4 cell lysate with PION antibody at 0.25 µg/mL in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry of PION in human brain tissue with PION antibody at 5 µg/mL.

Immunofluorescence of PION in Human Brain cells with PION antibody at 20 µg/mL.

Immunohistochemistry of PION in mouse brain tissue with PION antibody at 5 µg/mL.

Immunofluorescence of PION in mouse brain tissue with PION antibody at 20 µg/mL. Red: PION Antibody (orb1239874) Blue: DAPI staining.