Pig Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit

• Catalog Number: orb779572
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta 1(TGFb1).
• Reactivity: Porcine
• Concentration: 2000 pg/mL
• UniProt ID: P07200
• Tested applications: ELISA
• Application notes: standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig TGFb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig TGFb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig TGFb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig TGFb1 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Time: 3.5h
• Range: 31.25-2000 pg/mL
• Sensitivity: 12.3 pg/mL
• Sample Types: Serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids
• Alternative names: TGF Beta 1,TGF-B1 ELISA Kit, CED ELISA Kit, DPD1 E
• Note: For research use only