Pig Selectin, Platelet (SELP) ELISA Kit

SKU: orb779586

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Selectin, Platelet(SELP).

Research Area

Cancer Biology, Immunology & Inflammation, Infectious Disease & Virology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 400 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig SELP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig SELP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig SELP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig SELP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Porcine
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 6.25-400 ng/mL
• Sensitivity: 2.62 ng/mL
• Concentration: 400 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CD62P ELISA Kit, P-Selectin ELISA Kit, PSEL ELISA Kit, GMP140 ELISA Kit, LECAM3 ELISA Kit, GRMP ELISA Kit, GMRP ELISA Kit, PADGEM ELISA Kit, Leukocyte-endothelial cell adhesion molecule 3 ELISA Kit, Platelet activation dependent granule-external membrane ELISA Kit