Pig Procollagen III N-Terminal Propeptide (PIIINP) ELISA Kit

SKU: orb1088226

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Procollagen III N-Terminal Propeptide(PIIINP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Procollagen III N-Terminal Propeptide(PIIINP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Procollagen III N-Terminal Propeptide(PIIINP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Procollagen III N-Terminal Propeptide(PIIINP) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area

Gastroenterology & Hepatology, Metabolism Research, Musculoskeletal & Connective Tissue Research

Images & Validation

• Application Notes:
  standard: 400 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig PIIINP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig PIIINP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig PIIINP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig PIIINP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Porcine
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 6.25-400 ng/mL
• Sensitivity: 2.36 ng/mL
• Concentration: 400 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

P3NP, N-Propeptide Of Type III Procollagen, Procollagen III Amino Terminal Propeptide