You have no items in your shopping cart.
Cart summary
Item 1 of 1
| Catalog Number | orb780006 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Terminal Pro-Brain Natriuretic Peptide(NT-ProBNP). |
| Reactivity | Porcine |
| Concentration | 10 ng/mL |
| Tested applications | ELISA |
| Application notes | standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 0.16-10 ng/mL |
| Sensitivity | 0.065 ng/mL |
| Sample Types | Serum, plasma and other biological fluids |
| Alternative names | NT-Pro-BNP ELISA Kit, ELISA Kit, N-BNP ELISA Kit |
| Note | For research use only |
Pig NT-proBNP ELISA Kit [orb409677]
Porcine
7.8 pg/mL-500 pg/mL
1.95 pg/mL
24 T, 10 x 96 T, 5 x 96 T, 48 T, 96 T
