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Pig Monocyte Chemotactic Protein 2 (MCP2) ELISA Kit

Catalog Number: orb779775

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb779775
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Monocyte Chemotactic Protein 2(MCP2).
ReactivityPorcine
Concentration500 pg/mL
UniProt IDP49873
Tested applicationsELISA
Application notesstandard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig MCP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig MCP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig MCP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig MCP2 in the samples is then determined by comparing the OD of the samples to the standard curve
Assay TypeSandwich
Assay Time3.5h
Range7.82-500 pg/mL
Sensitivity3.2 pg/mL
Sample TypesSerum, plasma and other biological fluids.
Alternative namesCCL8 ELISA Kit, CCL-8 ELISA Kit, SCYA8 ELISA Kit,
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NoteFor research use only
Pig Monocyte Chemotactic Protein 2 (MCP2) ELISA Kit