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| Catalog Number | orb2667651 |
|---|---|
| Category | Assays and Kits |
| Description | Pig Matrix Metalloproteinase 9 (MMP9) ELISA Kit |
| Application notes | standard: 500 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig MMP9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig MMP9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig MMP9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig MMP9 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 7.81-500 ng/mL |
| Sensitivity | 1.38 ng/mL |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Alternative names | GELB; Gelatinase B; CLG4B; CLG4-B; 92 KDa Gelatina Read more... |
| Note | For research use only |
