Pig Macrophage Migration Inhibitory Factor (MIF) ELISA Kit

SKU: orb2667480

Description

The Pig Macrophage Migration Inhibitory Factor (MIF) ELISA Kit is designed to quantify Macrophage Migration Inhibitory Factor (MIF) in porcine serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 0.78-50 ng/mL and a sensitivity of 0.22 ng/mL.

Research Area

Neuroscience; Immunology & Inflammation

Images & Validation

• Application Notes:
  standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig MIF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig MIF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig MIF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig MIF in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Porcine
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.78-50 ng/mL
• Sensitivity: 0.22 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GIF; GLIF; MMIF; Glycosylation-Inhibiting Factor; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase

Quick Database Links

UniProt

P80928