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Catalog Number | orb781921 |
---|---|
Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Glucagon Like Peptide 2(GLP2) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glucagon Like Peptide 2(GLP2). |
Tested applications | ELISA |
Reactivity | Porcine |
Range | 156.25-10000 pg/mL |
Assay Time | 2.5h |
Concentration | 10000 pg/mL |
Sensitivity | 49.1 pg/mL |
Alternative names | Glp2, GLP2 Read more... |
Note | For research use only |
Application notes | standard: 10000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig GLP2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig GLP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig GLP2 in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Expiration Date | Please enquire. |
Porcine | |
125 pg/mL-2000 pg/mL | |
62.5 pg/mL |
Porcine | |
2.4 pg/mL-150 pg/mL | |
0.6 pg/mL |