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Catalog Number | orb1496518 |
---|---|
Category | Assays and Kits |
Description | Pig GC ELISA Kit |
Reactivity | Porcine |
Range | 31.25-2000 pg/mL |
Assay Time | 2.5h |
Concentration | 2000 pg/mL |
UniProt ID | P01274 |
Sensitivity | 11.31 pg/mL |
Alternative names | GCG; GLP1; GLP2; GRPP; Glicentin-Related Polypepti Read more... |
Note | For research use only |
Application notes | standard: 2000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig GC protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig GC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig GC in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | serum, plasma and other biological fluids |
Expiration Date | Please enquire. |
Porcine | |
125 pg/mL-2000 pg/mL | |
62.5 pg/mL |