Pig Diamine Oxidase (DAO) ELISA Kit

• Catalog Number: orb779476
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Diamine Oxidase(DAO).
• Reactivity: Porcine
• Concentration: 200 ng/mL
• UniProt ID: P00371
• Tested applications: ELISA
• Application notes: standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig DAO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig DAO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig DAO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig DAO in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 3.13-200 ng/mL
• Sensitivity: 1.12 ng/mL
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Alternative names: AOC1 ELISA Kit, ABP1 ELISA Kit, KAO ELISA Kit, His
• Research Area: Protein Biochemistry
• Note: For research use only