Pig Cystatin C (Cys-C) ELISA Kit

• Catalog Number: orb779547
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cystatin 3(CST3).
• Reactivity: Porcine
• Concentration: 800 ng/mL
• Tested applications: ELISA
• Application notes: standard: 800 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig Cys-C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig Cys-C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig Cys-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Time: 3.5h
• Range: 12.5-800 ng/mL
• Sensitivity: 5.8 ng/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: Cys-C ELISA Kit, Cystatin C ELISA Kit, Gamma Trace
• Note: For research use only