Pig Aprotinin (AP) ELISA Kit

SKU: orb779762

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Aprotinin(AP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Aprotinin(AP).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig AP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig AP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig AP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Porcine
• Sample Types: Serum, plasma and other biological fluids.
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.46 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

BPTI ELISA Kit, Trasylol ELISA Kit, Pancreatic Trypsin Inhibitor ELISA Kit, Basic protease inhibitor ELISA Kit