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| Catalog Number | orb779724 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiogenin(ANG). |
| Reactivity | Porcine |
| Concentration | 40 ng/mL |
| UniProt ID | P31346 |
| Tested applications | ELISA |
| Application notes | standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig ANG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig ANG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig ANG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig ANG in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 0.63-40 ng/mL |
| Sensitivity | 0.269 ng/mL |
| Sample Types | Serum, plasma and other biological fluids. |
| Alternative names | RNASE5 ELISA Kit, Ribonuclease,RNase A Family 5 EL Read more... |
| Note | For research use only |
Pig Angiotensinogen ELISA Kit [orb409579]
Porcine
94 pg/mL-6000 pg/mL
47 pg/mL
48 T, 24 T, 10 x 96 T, 96 T, 5 x 96 T
