PIAS1 Antibody

SKU: orb614004

Description

PIAS1 antibody

Images & Validation

• Tested Applications: DOT, ELISA, WB
• Dilution Range: ELISA: 5 µg/ml, WB: 1:1000
• Reactivity: Human
• Application Notes:
  Anti-PIAS1 Antibody has been tested for use in ELISA, Western Blotting, and Dot Blotting. HEK293 WCL p/n (W09-000-365) and K-562 WCL p/n (W09-001-GJ7) were used as positive control lysates in western blot. Expect a band at approximately 72 kDa in Western Blots of specific cell lysates and tissues. Specific conditions for reactivity should be optimized by the end user. While not tested, it is likely Anti-PIAS1 antibody will work for immunofluorescence, IHC, and CHIP assays.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-PIAS1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide from the internal region of human PIAS1.
• Purity: Anti-PIAS1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest reactivity to mouse 92.9% homology for the immunogen sequence. Cross-reactivity from PIAS1 family and other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

Rabbit anti-PIAS1 Antibody, Protein Inhibitor Of Activated STAT 1, GBP, ZMIZ3, DDXBP1, DEAD/H (Asp-Glu-Ala-Asp/His) Box Binding Protein 1, GU/RH-II, E3 SUMO-protein ligase PIAS1, Gu-binding protein, GBP, AR Interacting Protein

Western Blot of PIAS1 antibody. Lane 1: MW ladder Opal pre-stained. Lane 2: HEK293 whole cell lysate (p/n orb348669). Lane 3: K-562 whole cell lysate (p/n orb348692). Primary Antibody: Anti-PIAS1 antibody at 1.0 µg/ml. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:70000 for 45 min at RT. Block: orb348637 overnight at 4°C. Predicted/Observed size: 72 kDa, 60 kDa for PIAS1. Other band(s): PIAS1 splice variants and isoforms.

Quick Database Links

UniProt

O75925

RefSeq (Protein):NP_057250