phosphotyrosine antibody

• Catalog Number: orb345264
• Category: Antibodies
• Description: phosphotyrosine antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 13F9
• Tested applications: ELISA, WB
• Reactivity: Other
• Isotype: ASCITES
• Immunogen: This monoclonal antibody was produced after repeated immunizations of balb/c mice with phosphotyrosine conjugated KLH.
• Dilution range: ELISA: 1:4,000 - 1:20,000, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product is clarified ascites produced in balb/c mice using clone 13F9. Reactivity is specific for phosphotyrosine and minimal cross reactivity is observed against phosphoserine or phosphothreonine.
• Conjugation: Unconjugated
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-Phosphotyrosine Antibody, mouse anti-pY
• Note: For research use only
• Application notes: This monoclonal antibody has been tested in ELISA and Western Blot and reacts specifically with phosphotyrosine and shows minimal reactivity by ELISA and competitive ELISA with phosphoserine or phosphothreonine. The antibody reacts with free phosphotyrosine, phosphotyrosine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry, immunohistochemistry and immunoprecipitation. Phosphorylation of tyrosine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation.
• Expiration Date: 12 months from date of receipt.

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of using phosphotyrosine antibody

Western blot analysis of A431 cell lysate using phosphotyrosine antibody