Phosphotyrosine Antibody

SKU: orb345264

Description

phosphotyrosine antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:4,000 - 1:20,000, WB: 1:500 - 1:2,000
• Reactivity: Other
• Application Notes:
  This monoclonal antibody has been tested in ELISA and Western Blot and reacts specifically with phosphotyrosine and shows minimal reactivity by ELISA and competitive ELISA with phosphoserine or phosphothreonine. The antibody reacts with free phosphotyrosine, phosphotyrosine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry, immunohistochemistry and immunoprecipitation. Phosphorylation of tyrosine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: ASCITES
• Clone No.: 13F9
• Immunogen: This monoclonal antibody was produced after repeated immunizations of balb/c mice with phosphotyrosine conjugated KLH.
• Purity: This product is clarified ascites produced in balb/c mice using clone 13F9. Reactivity is specific for phosphotyrosine and minimal cross reactivity is observed against phosphoserine or phosphothreonine.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None. None
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-Phosphotyrosine Antibody, mouse anti-pY antibody, PhosphoTyrosine reactive antibody, monoclonal phosphotyrosine, anti-phosphotyrosine produced in mouse

ELISA of Mouse anti-phosphotyrosine antibody. Antigen: BSA conjugates of pT, pY and pS. Coating amount: 0.1 µg per well. Primary antibody: Phosphotyrosine antibody at 1:1000. Dilution series (X-axis): 2-fold. Secondary antibody: HRP conjugated Gt-a-Mouse IgG H&L secondary antibody at 1:20000. Substrate: TMB.

Western Blot of Mouse anti-phosphotyrosine antibody (clone 13F9). Lane 1: phosphorylated EGFR in an A431 cell lysate. Lane 2: total protein in the unstimulated A431 cells lysate after Coomassie staining. Load: 10 µg per lane. Primary antibody: phosphotyrosine antibody at 1:1000 for 1 hr at RT. Secondary antibody: IRDye™800 Conjugated Goat-a-Mouse IgG [H&L] diluted 1:2500 for 30' at room temperature. Block: 5% BLOTTO overnight at 4°C. Other band(s): phosphotyrosine splice variants and isoforms.