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AKT1/AKT2/AKT3 Antibody

SKU: orb572364

Description

This Phospho-AKT1/AKT2/AKT3 (Tyr315/316/312) Antibody is an unconjugated polyclonal product. It tagets AKT1/AKT2/AKT3 using a Peptide sequence around phosphorylation site of tyrosine 315/316/312 (P-E-Y(p)-L-A) derived from Human AKT1/AKT2/AKT3 as the immunogen. This antibody is suitable for ELISA, IF, IHC, WB.

Research Area

Neuroscience

Images & Validation

Tested ApplicationsELISA, IF, IHC, WB
Dilution RangeWB: 1:500-1:1000, IHC-P: 1:50-1:100, IF: 1:100-1:200
ReactivityHuman, Mouse, Rat

Key Properties

HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenPeptide sequence around phosphorylation site of tyrosine 315/316/312 (P-E-Y(p)-L-A) derived from Human AKT1/AKT2/AKT3.
TargetAKT1/AKT2/AKT3
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearanceliquid
Buffer/PreservativesSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

RAC-PK-alpha; Protein kinase B;

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

UniProt Details

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

AKT1/AKT2/AKT3 Antibody (orb572364)

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Available Sizes

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100 μl
$ 380.00
DispatchUsually dispatched within 5-10 working days
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