Phi29 DNA Polymerase

SKU: orb1733676

Description

Phi29 DNA polymerase is a recombinant protein purified from E.coli cloned the gene encoding the DNA polymerase from Phi29 phage. Phi29 DNA polymerase is the replicative polymerase from the Bacillus subtilis phage Phi29 and possesses the highest processivity and strand-displacement activity among the known DNA polymerase. Phi29 DNA polymerase contains a 3'→5' exonuclease activity that enables proofreading capability.

Research Area

Molecular Biology

Images & Validation

• Application Notes:
  Rolling-circle amplification (RCA); Multiple displacement amplification (MDA); Whole genome amplification (WGA); Preparation of DNA template for sequencing. ; Inactivation: Heat the mixture to 65 °C for 15 min.

Storage & Handling

• Storage: store at -20 °C. avoid freeze/thaw cycles
• Form/Appearance: liquid
• Concentration: 10 units/µl
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Bacillus subtilis phage phi29, recombinant, E. coli