PHB2 Antibody

• Catalog Number: orb1263351
• Category: Antibodies
• Description: PHB2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Bovine, Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This PHB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-255 amino acids from human PHB2.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 33 kDa
• Target: PHB2
• UniProt ID: Q99623
• NCBI: Q99623
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Prohibitin-2, B-cell receptor-associated protein B
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PHB2-Y248in MDA-MB435 cell line lysates (35 ug/lane)

Western blot analysis of PHB2-Y248 in mouse kidney tissue lysates (35 ug/lane)

Western blot analysis of PHB2 using PHB2 Antibody (Y248) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHB2 gene (Lane 2).

PHB2 Antibody (Y248) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PHB2 Antibody (Y248) for immunohistochemistry.