Pentoxifylline

SKU: orb1224349

Description

A METHYLXANTHINE derivative that inhibits phosphodiesterase and affects blood rheology. It improves blood flow by increasing erythrocyte and leukocyte flexibility. It also inhibits platelet aggregation. Pentoxifylline modulates immunologic activity by stimulating cytokine production. (In Vitro):Pentoxifylline (0.1-50 mM; 24-48 hours) inhibits cell proliferation in a dose-dependent manner.Pentoxifylline (0.5 mM; 12-36 hours) increases apoptosis and decreases autophagy levels in MDA-MB-231 cells.Pentoxifylline (0.5 mM; 12-36 hours) induces autophagy in MDA-MB-231 cells.Pentoxifylline (0.5 mM; 24-48 hours) blocks cell cycle at the G0/G1 phase.Pentoxifylline results in high LC3-II/LC3-ratio.(In Vivo):Pentoxifylline (200 mg/kg; i.p.) has a protective effect on rats with transient global ischemia and reduces cognitive impairment.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 6493-05-6
• MW: 278.31
• Purity: >98% (HPLC)
• Formula: C₁₃H₁₈N₄O₃
• SMILES: c12c(n(c(=O)n(c1=O)CCCCC(C)=O)C)ncn2C
• Target: Adenosine Receptor
• Solubility: soluble in Water

Bioactivity

• In Vivo:
  Pentoxifylline (200 mg/kg; i.p.) has a protective effect on rats with transient global ischemia and reduces cognitive impairment. Animal model: Adult male Wistar rats 12-13-weeks-old (250-300 g). Dosage: 200 mg/kg. Administration: Intraperitoneal injection, at 1hr before and 3 hr after ischemia. Result: Significantly improved the spatial memory and effects were significant different from those of sham-operated and vehicle groups.
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• In Vitro:
  Pentoxifylline (0.1-50 mM; 24-48 hours) inhibits cell proliferation in a dose-dependent manner. Pentoxifylline (0.5 mM; 12-36 hours) increases apoptosis and decreases autophagy levels in MDA-MB-231 cells. Pentoxifylline (0.5 mM; 12-36 hours) induces autophagy in MDA-MB-231 cells. Pentoxifylline (0.5 mM; 24-48 hours) blocks cell cycle at the G0/G1 phase. Pentoxifylline results in high LC3-II/LC3-ratio. Cell Proliferation Assay Cell line: MDA-MB-231 cells. Concentration: 0.1 mM, 1 mM, 5 mM, 10 mM, 50 mM Incubation time: 24 hours, 48 hours. Result: Inhibited cell proliferation in a dose-dependent manner. Apoptosis Analysis Cell line: MDA-MB-231 cells. Concentration: 0.5 mM Incubation time: 12 hours, 24 hours, 36 hours. Result: Induced apoptosis. Cell Autophagy Assay Cell line: MDA-MB-231 cells. Concentration: 0.5 mM Incubation time: 24 hours, 48 hours. Result: Induced approximately 20-28% of cell autophagy. Cell Cycle Analysis Cell line: MDA-MB-231 cells. Concentration: 0.5 mM Incubation time: 24 hours, 48 hours. Result: Induced G0/G1 phase arrest. Western blot analysis. Cell line: MDA-MB-231 cells. Concentration: 0.5 mM Incubation time: 24 hours, 48 hours. Result: Induced high LC3-II/LC3-ratio.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NSC 637086 | Oxpentifylline

Quick Database Links

Gene Symbol

Adenosine Receptor