Pemetrexed disodium hemipenta hydrate

SKU: orb1300961

Description

Pemetrexed disodium hemipenta hydrate

Research Area

Cell Biology, Metabolism Research, Molecular Biology

Key Properties

• CAS Number: 357166-30-4
• MW: 518.43
• Purity: 98.76%
• Formula: C₂₀H₂₁N₅O₆·2Na·5/2H₂O
• SMILES: O.O.O.O.O.[Na+].[Na+].[Na+].[Na+].Nc1nc(=O)c2c(CCc3ccc(cc3)C(=O)N[C@@H](CCC([O-])=O)C([O-])=O)c[nH]c2[nH]1.Nc1nc(=O)c2c(CCc3ccc(cc3)C(=O)N[C@@H](CCC([O-])=O)C([O-])=O)c[nH]c2[nH]1
• Target: Autophagy,Antifolate,DHFR,DNA/RNA Synthesis,Apoptosis
• Solubility: H2O:100 mg/mL (192.89 mM);DMSO:Slightly soluble

Bioactivity

• Target IC50:
  GARFT:65 nM(Ki)|TS:1.3 nM(Ki)|DHFR:7.2 nM(Ki)
• In Vivo:
  In CCRF-CEM leukemia, GC3/C1 colon cancer, and HCT-8 ileocecal colon cancer cells, Pemetrexed disodium exhibited antitumor activity with IC50 Values of 25 nM, 34 nM, and 220 nM, respectively
• In Vitro:
  I the human H460 non-s All cell lung carcinoma xenograft model, Pemetrexed disodium effectively inhibits tumor growth.
• Cell Research:
  Dose-response curves are generated to determin the concentration required for 50% Inhibition of growth IC50 . Pemetrexed disodium is dissolved initially in DMSO at a concentration of 4 mg mL and further diluted with cell culture medium to the desired concentration CCRF-CEM leukemia cells in complete medium are added to 24-well Cluster plates in a total volume of 2.0 mL. Pemetrexed disodium at various concentration are added to duplicate wells so tha the final volume of DMSO is 0.5% the plates are incubated for 72 hour at 37℃n an atmosphere of 5% CO2 in air. At the end of the incubation, cell numbers are determined on a ZBI Coulter counter. For several studies, IC50 are determined for Each compound in the presence of either 300 μM AICA, 5 μM thymidine, 100 μM hypoxanthine, or combination of 5 μM hymidine plus 100 μM hypoxanthine. For adherent tumor cells, a modification o the original MTT colorimetric assay is used to measure cell cytotoxicity the human tumor cells are seeded in 100 μL assay medium/well in 96-well flat-bottomed tissue culture plates the assay medium contains folic acid-free RPMI 1640 supplemented with 10% FCS and either 2 nM folinic acid or 2.3 μM folic acid a the sole folate source. Well 1A is left blank. Stock solutions of antifolates are prepared in Dulbecco's PBS at 1 mg/mL, and a series of 2-fold dilutions are Subsequently made in PBS. Ten-μL aliquots of Each concentration are added to triplicate wells. Plates are incubated for 72 hours at 37℃n a humidified atmosphere of 5% CO2-in-air. MTT is dissolved in PBS at 5 mg/mL, 10 μL of stock MTF solution are added to each well of an assay, an the plates are incubated at 37℃or 2 additional hours. Following incubation, 100 μL of DMSO are added to Each well. After thorough formazan solubilization the plates are read on a Dynatech MR600 reader, using a test wavelength of 570 nM and a reference wavelength of 630 nM.The IC50 is determined a the concentration of drug required to inhibit cell growth by 50% compared to an untreated controls.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DHFR, DNA Synthesis, DNASynthesis, Apoptosis, Antifolate, Autophagy, Inhibitor, GARFT, LY231514 disodium hemipenta, LY-231514 Disodium Hydrate, inhibit, RNA Synthesis, RNASynthesis, Pemetrexed sodium Hydrate, Pemetrexed disodium hemipenta, Pemetrexed disodium hemipenta hydrate, Pemetrexed disodium hemipenta Hydrate, Pemetrexed sodium hydrate, Pemetrexed Disodium Hydrate, Thymidylate synthase

Compound Identifiers

PubChem CID

135564527