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Description
Research Area
Cancer Biology, Cell Biology, Immunology & Inflammation, Metabolism Research, Signal Transduction
Images & Validation
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Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | The ED50 was determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is ≤ 1 ng/mL, corresponding to a specific activity of ≥ 1 x 106 units/mg. |
| Target | PDGFA |
| Purity | Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
| Protein Sequence | Alpha Chain: SIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPTBeta Chain: SLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIGIVR KKPIFKKATV TLGDHLACKC ETVAAARPVT |
Storage & Handling
−| Storage | The lyophilized PDGF-AB recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted PDGF-AB is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing PDGF-AB recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−PDGF1, PDGF-A, PDGF1, Platelet-derived growth factor subunit A, PDGF-1, PDGF subunit A
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Recombinant human PDGF-AB protein (Active, CHO) [orb2978610]
≥ 95% as determined by SDS-PAGE.
14.3 &12.3 kDa
10 μg, 50 μg, 500 μg
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_002598.4 |
|---|
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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