Pdcd4 (phospho-S457) antibody

• Catalog Number: orb344433
• Category: Antibodies
• Description: Pdcd4 (phospho-S457) antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 9G6
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG1
• Immunogen: This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Ser457 of the human Pdcd4 protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Pdcd4 phospho S457 Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human Pdcd4 protein phosphorylated at Ser457. A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q53EL6
• NCBI: 21735596
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-Pdcd4 pS457 Antibody, Death up-regulate
• Note: For research use only
• Application notes: Pdcd4 phospho S457 Antibody is tested by ELISA, immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 62 kDa in size corresponding to phosphorylated Pdcd4 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human Pdcd4 pS457 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of human tissues using Pdcd4 (phospho-S457) antibody

Western blot analysis of NIH-3T3 cells using Pdcd4 (phospho-S457) antibody