Pdcd4 phospho S457 Antibody

SKU: orb344433

Description

Pdcd4 (phospho-S457) antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Reactivity: Human, Mouse
• Application Notes:
  Pdcd4 phospho S457 Antibody is tested by ELISA, immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 62 kDa in size corresponding to phosphorylated Pdcd4 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human Pdcd4 pS457 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 9G6
• Immunogen: This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Ser457 of the human Pdcd4 protein.
• Purity: Pdcd4 phospho S457 Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human Pdcd4 protein phosphorylated at Ser457. A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-Pdcd4 pS457 Antibody, Death up-regulated gene protein antibody, Dug antibody, H731 antibody, Ma3 antibody, Neoplastic transformation inhibitor antibody, Neoplastic transformation inhibitor protein antibody, Nuclear antigen H731 antibody

Biorbyt Antibody orb344433 has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The antibody was serially diluted and tested at a range of concentrations on at least 22 different human formalin-fixed, paraffin archival tissues, and positive and negative tissues were scored and compared to the published literature on the expression and function of the gene. A representative image from positively stained small intestine shows the localization of the anti Pdcd4 antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Western blot using Biorbyt's Protein A purified Mouse Monoclonal anti-Pdcd4 pS457 antibody against recombinant PDCD4 protein. Membrane was blocked in 1% BSA-TBS-T for 30 min RT and probed with 1°Ab Ms-A-Pdcd4pS457 1:1000 (o/n 4°C in 1% BSA-TBS-T) followed by 2°Ab Peroxidase Conjugated Rabbit anti-Ms CUST10M at 1:40000 in orb348637 30 min RT. Bands at ~62 kD and ~32 kD were detected.

Western blot using Biorbyt's Protein A purified Mouse Monoclonal anti-Pdcd4 pS457 antibody shows detection of phosphorylated Pdcd4 (indicated by arrowhead at ~62 kDa) in NIH-3T3 cells (p/n orb348723) after 5 min treatment with 30 ng/mL PDGF [lane 2]. No reactivity is seen for (non-phosphorylated) in unstimulated NIH 3T3 cells (p/n orb348714) [lane 1]. The membrane was probed with the primary antibody at a 1:2000 dilution, overnight at 4°C. For detection HRP conjugated Rb-a-Mouse IgG (p/n orb347544) was used at a 1:20000 dilution in blocking buffer (p/n orb348637) for 1 h at 4°C.

Quick Database Links

UniProt

Q53EL6

RefSeq:21735596