PD98059

SKU: orb1225975

Description

PD98059 (PD 098059) is a specific MEK inhibitor with IC50 of ~10 uM without significant inhibitory activity of MAPK; prevents activation of MAPK and subsequent phosphorylation of MAPK substrates both in vitro and in intact cells.(In Vitro):PD98059 (20 μM; 24 hours) causes G1-phase cell cycle arrest in OCI-AML-3 cells.PD98059 (10 μM; 22 hours) results in concentration-dependent reductions in the dually phosphorylated forms of ERK1 and ERK2. PD98059 both prevents ERK activation and blocks formation of TDP-43 and HuR-positive SGs.(In Vivo):PD98059 (10 mg/kg; i.p.; 1 and 6 hours after Zymosan) significantly reduces the level of p-ERK1/2 in zymosan-injected mice.

Key Properties

• CAS Number: 167869-21-8
• MW: 267.2793
• Purity: >98% (HPLC)
• Formula: C₁₆H₁₃NO₃
• SMILES: O=C1C=C(C2=CC=CC(OC)=C2N)OC3=C1C=CC=C3
• Target: MEK
• Solubility: DMSO: 16 mg/mL; H2O: < 0.01 mg/mL

Bioactivity

• In Vivo:
  Animal model: Male CD mice. Dosage: 10 mg/kg. Administration: Intraperitoneal injection; 1 and 6 hours after Zymosan. Result: Significantly reduced the level of p-ERK1/2.
• In Vitro:
  Cell Cycle Analysis Cell line: OCI-AML-3 cells. Concentration: 20 μM. Incubation time: 24 hours. Result: Caused G1-phase cell cycle arrest. Western blot analysis. Cell line: MCF10A-Neo, MCF10ANeoT cells. Concentration: 10 μM. Incubation time: 22 hours. Result: Phosphorylated ERK forms were almost completely eliminated in both cell lines.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PD 098059 | PD-98059

Quick Database Links

Gene Symbol

MEK