PD173955

SKU: orb1304576

Description

PD173955

Research Area

Cardiovascular Research, Cell Biology, Signal Transduction

Key Properties

• CAS Number: 260415-63-2
• MW: 443.35
• Purity: 98.52%
• Formula: C₂₁H₁₆Cl₂N₄OS
• SMILES: CSc1cccc(Nc2ncc3cc(-c4c(Cl)cccc4Cl)c(=O)n(C)c3n2)c1
• Target: Src,Bcr-Abl,Apoptosis
• Solubility: DMSO:12 mg/mL (27.07 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (4.51 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  Src:22 nM
• In Vivo:
  PD173955 serves as a Src inhibitor, effectively inhibiting mitotic progression during the early stages of mitosis across various cell types while inducing apoptosis to varying degrees. It efficiently suppresses Bcr-Abl-dependent cellular growth, displaying an IC50 value of 2-35 nM in Bcr-Abl-positive cell lines, which is approximately 100 to 200 times more sensitive than in Bcr-Abl-negative cell lines. Additionally, PD173955 inhibits the stem cell factor-dependent proliferation of M07e cells by blocking the ligand-dependent autophosphorylation of c-kit, with an IC50 of 40 nM.
• Cell Research:
  Cell growth is determined by two methods. For the [3H]thymidine assay, cells (104 cells/well) are cultured in 96-well, round-bottomed plates with diluted DMSO (control) or with varying concentrations of a specific compound that is resuspended in DMSO for 48 h at 37°C. [3H]Thymidine is added at a concentration of 1 μCi/well, and cells are incubated for an additional 18 h. Cells were harvested with the Unifilter system, scintillation fluid (25 μl/well) is added to each well, and [3H]thymidine incorporation is determined on a Packard Scintillation Counter. Data points for all assays are obtained in triplicate, and background incorporation from cell-free wells is determined and subtracted from all data points. For cell viability, control and drug-treated harvested cells are counted on a hemocytometer using the trypan blue dye exclusion method.(Only for Reference)

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Src, PD173955, PD-173955, PD 173955, inhibit, Inhibitor, Apoptosis, Bcr-Abl, BcrAbl

Compound Identifiers

PubChem CID

447077