PBIT

SKU: orb1219281

Description

PBIT inhibits JARID1B histone demethylase (IC50 of about 3 μM). PBIT also inhibits JARID1A and JARID1C (IC50s of 6 μM and 4.9 μM, respectively). PBIT is a specific inhibitor of the Jumonji AT-rich Interactive Domain 1 (JARID1) enzymes . PBIT, which inhibits JARID1B with an IC50 of about 3 μm in vitro. Consistent with this, PBIT treatment inhibited removal of H3K4me3 by JARID1B in cells. Furthermore, this compound inhibited proliferation of cells expressing higher levels of JARID1B.

Key Properties

• CAS Number: 2514-30-9
• MW: 241.31
• Purity: >98% (HPLC)
• Formula: C₁₄H₁₁NOS
• SMILES: O=C1N(C2=CC=C(C)C=C2)SC3=C1C=CC=C3
• Target: JARID1B|JARID1A|JARID1C
• Solubility: DMSO:100 mg/mL (414.40 mM; Need ultrasonic)

Bioactivity

• In Vitro:
  Cell Proliferation Assay Cell line: Human breast cancer cell lines (UACC-812 and MCF7) and human mammary epithelial cells (MCF10A). Concentration: 1, 3, and 10 μM for UACC-812 cells; 2.5, 5, and 10 μM for MCF7 and MCF10A cells Incubation time: 72 hours. Result: Inhibited cell proliferation in a JARID1B level-dependent manner. 10 μM killed most of the UACC-812 cells, but showed minimal toxicity to MCF7 cells and MCF10A cells.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

JARID1B|JARID1A|JARID1C