Pan-AKT Antibody

• Catalog Number: orb1258466
• Category: Antibodies
• Description: Pan-AKT Antibody
• Target: AKT1/AKT2/AKT3
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Immunogen: A synthetic peptide of human AKT1/AKT2/AKT3
• UniProt ID: P31751, Q9Y243, P31749
• MW: Observed: 60kDa
• Tested applications: IF, IHC, WB
• Application notes: WB: 1:500 - 1:1000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using AKT1/AKT2/AKT3 antibody (orb1258466) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.