Pamidronic acid

SKU: orb1745286

Description

Pamidronic acid is a bisphosphonate compound used in research to study osteonecrosis mechanisms. It inhibits osteogenic differentiation in human bone marrow mesenchymal stem cells by suppressing Wnt/β-catenin signaling, a pathway critical for bone formation. This makes it a valuable tool for in vitro models of bone metabolism disorders and drug-induced osteonecrosis.

Research Area

Immunology & Inflammation, Infectious Disease & Virology, Pharmacology & Drug Discovery, Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 40391-99-9
• MW: 235.07
• Purity: 98.00% (May vary between batches)
• Formula: C₃H₁₁NO₇P₂
• SMILES: O.O.O.O.O.[Na+].[Na+].NCCC(O)(P(O)([O-])=O)P(O)([O-])=O
• Target: Wnt/beta-catenin,Antibacterial,RANKL/RANK,TLR
• Solubility: H2O:6 mg/mL (25.52 mM)

Bioactivity

• In Vitro:
  Pamidronate disodium inhibited Wnt and β-catenin signaling, which controls osteogenic differentiation in BMMSCs. Wnt3a, a Wnt and β-catenin signaling activator, reversed the negative effects caused by pamidronate disodium to salvage the osteogenic defect in BMMSCs.
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• Cell Research:
  Primary human BMMSCs were isolated from the mandible and marrow tissue. A proliferation assay was performed to determine the experimental concentration of pamidronate disodium. Alkaline phosphatase (ALP) activity, ALP staining, and Alizarin red S (ARS) staining were assessed after treatment with pamidronate disodium (0, 0.1, 0.5, 1, 5, 10 μg/mL). Quantitative real-time polymerase chain reaction and western blotting specific for Wnt and β-catenin signaling genes or proteins were performed after treatment with pamidronate disodium 0.5 μg/mL. Wnt3a was used to observe the osteogenic differentiation of BMMSCs during treatment with pamidronate disodium 0.5 μg/mL.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, Pamidronic acid, inhibit, beta-catenin, betacatenin, Beta catenin, bcatenin, Wnt/β-catenin, Wnt, Wnt/betacatenin, Wnt/b-catenin, β-catenin, βcatenin

Compound Identifiers

PubChem CID

60342