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PAK1 Antibody

SKU: orb1247149

Description

PAK1 Antibody

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsELISA, WB
ReactivityHuman, Mouse, Rat
Predicted ReactivityCanine
Application Notes
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 65kDa band observed in Human Brain (Cerebral Cortex and Hippocampus) lysates (calculated MW of 60.6kDa according to NP_002567.3). Recommended concentration: 0.5-1.5µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-NTEKQKKKPKMSDE
TargetPAK1
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

PAK1, p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) , MGC130000, MGC130001, PAKalpha , p21-activated kinase 1, p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)

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PAK1 Antibody

orb1247149 (0.5 ug/ml) staining of Human Brain (Cerebral Cortex) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Documents Download

Datasheet
Product Information
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

PAK1 Antibody (orb1247149)

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0.1 mg
$ 920.00
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