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Description
Research Area
Cancer Biology, Cell Biology, Immunology & Inflammation, Metabolism Research, Signal Transduction
Images & Validation
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Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37˚C. Half maximal inhibition against 1.0 μg/mL of single chain tPA was obtained at a concentration of 2.0 μg/mL. |
| Target | SERPINE1 |
| Purity | Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
| Protein Sequence | MVHHPPSYVA HLASDFGVRV FQQVAQASKD RNVVFSPYGV ASVLAMLQLT TGGETQQQIQ AAMGFKIDDK GMAPALRHLY KELMGPWNKD EISTTDAIFV QRDLKLVQGF MPHFFRLFRS TVKQVDFSEV ERARFIINDW VKTHTKGMIS NLLGKGAVDQ LTRLVLVNAL YFNGQWKTPF PDSSTHRRLF HKSDGSTVSV PMMAQTNKFN YTEFTTPDGH YYDILELPYH GDTLSMFIAA PYEKEVPLSA LTNILSAQLI SHWKGNMTRL PRLLVLPKFS LETEVDLRKP LENLGMTDMF RQFQADFTSL SDQEPLHVAQ ALQKVKIEVN ESGTVASSST AVIVSARMAP EEIIMDRPFL FVVRHNPTGT VLFMGQVMEP |
Storage & Handling
−| Storage | The lyophilized PAI-1 recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted PAI-1 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing PAI-1 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−PAI, PAI1, PAI-1, PLANH1, Plasminogen activator inhibitor 1, Endothelial plasminogen activator inhibitor, PAI
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000593.1 |
|---|
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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