P4HA1 Antibody

• Catalog Number: orb1538026
• Category: Antibodies
• Description: P4HA1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IHC-P, WB
• Reactivity: Bovine, Canine, Equine, Gallus, Human, Mammal, Monkey, Porcine, Rabbit
• Immunogen: Peptide with sequence C-DFARKDEPDAFKE, from the internal region of the protein sequence according to NP_000908.2NP_001017962.1.
• Concentration: 0.5 mg/ml
• Dilution range: IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:32000), WB (0.1 µg/ml)
• Conjugation: Unconjugated
• Target: P4HA1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Alternative names: P4HA1, 4-PH alpha-1, C-P4Halpha(I), p4HA, p4HA1
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: LS-B3291 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3291 was determined to be 5 ug/ml.
• Expiration Date: 12 months from date of receipt.

Antibody (0.2 ug/ml) staining of HEK293 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.