P4HA1 Antibody

SKU: orb19434

Description

Goat polyclonal antibody to P4HA1

Images & Validation

• Tested Applications: ELISA, IF, IHC
• Dilution range: ELISA: 1:128000, IF/ICC: 10μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 60kDa band observed in lysates of cell lines HEK293, A431 and NIH3T3 (calculated MW of 61.0kDa according to NP_000908.2 and NP_001017962.1). Recommended concentration: 0.1-0.1 μg/ml. An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: P4HA1
• Molecular Weight: 61.0, 61.0
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti P4HA1 antibody, anti procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I antibody, anti 4-PH alpha-1 antibody, anti P4HA antibody, anti prolyl 4-hydroxylase, alpha I subunit antibody

Immunofluorescence analysis of A431 cells using P4H41 antibody.

Immunohistochemical staining of Human Spleen using P4HA1 antibody.

Immunohistochemical staining of Human Spleen using P4HA1 antibody.

Quick Database Links

Gene Symbol

P4HA1

NCBI Gene ID

5033