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Goat anti-P4HA1 Antibody

Catalog Number: orb19434

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb19434
CategoryAntibodies
DescriptionGoat polyclonal antibody to P4HA1
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, IF, IHC
ReactivityBovine, Canine, Human
Dilution rangeELISA: 1:128000, IF/ICC: 10μg/ml
ConjugationUnconjugated
MW61.0, 61.0
TargetP4HA1
Entrez5033
Protein SequenceDFARKDEPDAFKE
RRIDAB_10749916
StorageAliquot and store at -20°C. Minimize freezing and thawing.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesanti P4HA1 antibody, anti procollagen-proline, 2-o
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NoteFor research use only
Application notesELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 60kDa band observed in lysates of cell lines HEK293, A431 and NIH3T3 (calculated MW of 61.0kDa according to NP_000908.2 and NP_001017962.1). Recommended concentration: 0.1-0.1 μg/ml. An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Expiration Date12 months from date of receipt.
Goat anti-P4HA1 Antibody

Immunofluorescence analysis of A431 cells using P4H41 antibody.

Goat anti-P4HA1 Antibody

Immunohistochemical staining of Human Spleen using P4HA1 antibody.

Goat anti-P4HA1 Antibody

Immunohistochemical staining of Human Spleen using P4HA1 antibody.