p39 Control protein

SKU: orb345970

Description

p39 Control protein

Images & Validation

• Tested Applications: SDS-PAGE, WB
• Application Notes:
  p39 is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 77.8 kDa for p39-MBP, (35.4 kDa for p39 and 42.4 kDa for MBP) in size corresponding to p39 by Western blotting in the appropriate cell lysate or extract. p39 protein was tested in SDS-page and western blot.

Key Properties

• Source: Borrelia burgdorferi
• Expression System: E. coli
• Biological Origin: Borrelia burgdorferi
• Purity: p39 is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified p39 and was estimated to be greater than 90% pure.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 0.994mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

Basic membrane protein A, Borrelia burgdorferi bmpA, immunodominant antigen P39, membrane lipoprotein BmpA, control protein

Antigen-specific IgG2, IgA1, and ADCD partitioning between compartments differs significantly across disease phenotypes. (A) Fraction of samples with non-zero measurements for ADCD, IgG1, IgG2, IgG3, and IgA1 for refractory (dark blue) and responsive (green) patients in the serum (dashed line) and joint fluid (solid line) for each antigen. Significant differences in distribution of non-zero measurements between fluids as assessed by a Fisher's exact test are denoted as *p < 0.05, **p < 0.01, ***p < 0.001 for refractory (dark blue) and responsive (green) samples after correction for multiple hypothesis testing via Benjamini-Hochburg. (B) Ratio of fraction of serum samples with non-zero measurements to fraction of joint fluid samples with non-zero measurements for ADCD, IgG1, IgG2, IgG3, and IgA1 for refractory (dark blue) and responsive (green) patients for each antigen. Significant differences in distributions of ratios between phenotypes are assessed by a Mann-Whitney nonparametric test, then corrected for multiple hypothesis testing via Benjamini-Hochburg. CRASP1, CRASP2, DbpA, DbpB, Arp37, flagellin, OspA, OspB, OspC, OspE, p27, p35, p39, VlsE: Biorbyt antigens.

SDS-PAGE Results of p35 Control Protein. Lane 1: p39 Control Protein Reduced [5.0 µg]. Lane 2: p39 Control Protein Reduced [1.0 µg]. Lane 3: Opal Prestained Molecular Weight Marker. Lane 4: p39 Control Protein Non-Reduced [5.0 µg]. Lane 5: p39 Control Protein Non-Reduced [1.0 µg]. 4-20% gel, Coomassie stained. Expected MW of p39-MBP ~77.8kDa.

Systems serology profiling with Borrelia-specific antigens reveals patient heterogeneity. The heatmap shows the Z-scored measurements for 12 features, across 16 antigens for both refractory and responsive patients, visualized with joint fluid measurements in the upper half of the heatmap and serum measurements in the lower half of the heatmap. Only antigens detected above background for at least 30% of samples were included for each measurement. Statistical significance was assessed using the Mann-Whitney nonparametric test, with p values then corrected for multiple hypothesis testing via Benjamini-Hochburg, *p < 0.05, **p < 0.01, ***p < 0.001, else not significant. CRASP1 (p/n orb345971), CRASP2 (p/n orb345972), DbpA (p/n orb345961), DbpB (p/n orb345969), Arp37 (p/n orb345962), flagellin (p/n orb345967), OspA (p/n orb345966), OspB (p/n orb345968), OspC (p/n orb345964), OspE (p/n orb345963), p27 (p/n orb345973), p35 (p/n orb345965), p39 (p/n orb345970), VlsE (p/n orb345974).

Western Blot of p35 Control Protein. Lane 1: p39 Control Protein (p/n orb345970) Reduced [0.1 µg]. Lane 2: p39 Control Protein (p/n orb345970) Reduced [0.2 µg]. Lane 3: Opal Prestained Molecular Weight Marker. Lane 4: p39 Control Protein (p/n orb345970) Non-Reduced [0.1 µg]. Lane 5: p39 Control Protein (p/n orb345970) Non-Reduced [0.2 µg]. Primary Antibody: Anti-p39 (p/n orb344680) at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat anti-Rabbit IgG [H&L] HRP (p/n orb347673) at 1:40000 for 30 mins at RT. Blocking: BlockOut Buffer (orb348644) for 1hr RT. Expected MW: 77.8kDa. Exp: 10sec.

Quick Database Links

UniProt

Q45010

RefSeq (Protein):WP_002656850.1