OTUB2-IN-1

SKU: orb2564745

Description

OTUB2-IN-1 is a selective OTUB2 inhibitor that suppresses PD-L1 protein expression in tumor cells, demonstrating anti-tumor activity. It has been utilized in research models, including in vitro cell studies and in vivo animal models, for investigating cancers such as melanoma and non-small cell lung cancer (NSCLC).

Research Area

Cell Biology, Molecular Biology, Protein Biochemistry

Key Properties

• MW: 434.49
• Formula: C₁₉H₁₈N₂O₆S₂
• SMILES: O=C(O)CN(C(/C1=C2C(N(CCCCCC(O)=O)C(S\2)=S)=O)=O)C3=C1C=CC=C3
• Target: DUB
• Solubility: DMSO:40 mg/mL (92.06 mM)

Bioactivity

• In Vivo:
  Evaluated the therapeutic potential of OTUB2-IN-1 using a mouse LL/2 tumor model. OTUB2-IN-1 treatment significantly suppressed tumor growth and prolonged mouse survival, and no obvious toxicity was observed. To clarify the effect of OTUB2-IN-1 in tumor inhibition through regulation of PD-L1 in vivo, LL/2 cells with stably expressed PD-L1 and control plasmids were used to establish a tumor model. As expected, in comparison to the control group, overexpressed PD-L1 resulted in a substantial increase in tumor growth, whereas OTUB2-IN-1 significantly reduced the PD-L1-overexpressing tumor growth.
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• In Vitro:
  Incubation of recombinant GST-OTUB2 protein with OTUB2-IN-1 resulted in significant inhibition of Ub-R110 cleavage by GST-OTUB2 in a dose-dependent manner, suggesting that OTUB2-IN-1 can inhibit the hydrolytic activity of recombinant GST-OTUB2 protein toward its substrate Ub-R110 in vitro.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

OTUB2, OTUB2IN1

Compound Identifiers

PubChem CID

3819376