OSMI-4

SKU: orb1708966

Description

OSMI-4 is a potent and cell-permeable O-GlcNAc transferase (OGT) inhibitor, exhibiting low nanomolar biochemical activity and a cellular EC50 of 3 µM. This small molecule is a valuable chemical probe for investigating O-GlcNAcylation in various human cell lines, supporting research in cellular signaling, metabolism, and related disease models.

Research Area

Metabolism Research

Key Properties

• CAS Number: 2260791-14-6
• MW: 604.09
• Purity: >99.99% (May vary between batches)
• Formula: C₂₇H₂₆ClN₃O₇S₂
• SMILES: CCOC(=O)CN(Cc1cccs1)C(=O)[C@H](NS(=O)(=O)c1cc2ccc(=O)[nH]c2cc1Cl)c1ccccc1OC
• Target: Others,Acyltransferase,Transferase
• Solubility: DMSO:252.5 mg/mL (417.98 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:5 mg/mL (8.28 mM)

Bioactivity

• Target IC50:
  OGT:3 μM (EC50)|OGT:60 nM
• In Vitro:
  METHODS: HEK293T cells were treated with OSMI-4 (10 μM, 2 hours) and quantitative PCR was performed using primers for the OGT retained intron (intron 4) and spliced ​​exon (exon 4). RESULTS The bar graph shows that OSMI-4 increases retained intron splicing. METHODS: HEK293T cells were treated with OSMI-4 (20, 50 μM, 24 hours); HEK293T cells were treated with OSMI-4 (0.1, 1, 5, 5, 10, 20 μM, 2, 24, 48 hours); Western blot analysis of total O-GlcNAc was performed. RESULTS O-GlcNAc levels were reduced to a greater extent following OSMI-4 treatment; 24 hours of OSMI-4 treatment of HEK293T cells resulted in a dose-dependent decrease in O-GlcNAc levels, with effects seen with only 5 µM of compound treatment, and these effects could be observed in as little as 2 hours or 48 hours.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

OSMI 4, OSMI4, OSMI-4, O-GlcNAc transferase (OGT)

Compound Identifiers

PubChem CID

137319721