OSMI-1

SKU: orb1218661

Description

OSMI-1 inhibits protein O-linked N-acetylglucosamine (O-GlcNAcylation) in several mammalian cell lines without qualitatively altering cell surface N- or O-linked glycans. OSMI-1 is a cell-permeable O-GlcNAc transferase (OGT) inhibitor (IC50: 2.7 μM).

Key Properties

• CAS Number: 1681056-61-0
• MW: 563.64
• Purity: >98% (HPLC)
• Formula: C₂₈H₂₅N₃O₆S₂
• SMILES: O=C(N(CC1=CC=CS1)CC2=CC=CO2)[C@H](NS(C3=CC(C=C4)=C(C=C3)NC4=O)(=O)=O)C5=CC=CC=C5OC
• Target: O-GlcNAc transferase (OGT)
• Solubility: DMSO:83.33 mg/mL (147.84 mM; Need ultrasonic)

Bioactivity

• In Vivo:
  Mammalian and zebrafish toxicity profiles are strikingly similar, and zebrafish can usually serve as an intermediate step between cell-based evaluation and conventional animal testing. The zebrafish model is used to investigate OSM1-1 acute toxicity In vivo. The LC50 of OSM1-1 is 0.031 mg/mL (56 μM, 12 h) and 0.025 mg/mL (45 μM, 24 h) in zebrafish model.
• In Vitro:
  OSMI-1 (50 μM; 24 hours; CHO cells) treatment decreases the viability by about 50% after 24 hours. OSMI-1 (10-100 μM; 24 hours; CHO cells) treatment reduces global O-linked N-acetylglucosamine (O-GlcNAcylation) a dose-dependent manner. OSMI-1 inhibits OGT activity in cells. Cell Viability Assay Cell line: CHO cells. Concentration: 50 μM. Incubation time: 24 hours. Result: Viability decreased by about 50% after 24 hours. Western blot analysis. Cell line: CHO cells. Concentration: 10 μM, 25 μM, 50 μM, 100 μM. Incubation time: 24 hours. Result: Reduced global OGlcNAcylation in a dose-dependent manner.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

O-GlcNAc transferase (OGT)