Oseltamivir phosphate

SKU: orb1308499

Description

Oseltamivir phosphate (GS 4104) is an orally active neuraminidase inhibitor used in research to impede influenza viral egress from host cells. It is widely applied in antiviral studies for both in vitro assays and in vivo animal models of influenza infection.

Research Area

Infectious Disease & Virology, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 204255-11-8
• MW: 410.4
• Purity: >98%
• Formula: C₁₆H₂₈N₂O₄•H₃PO₄
• SMILES: [C@H]1([C@H]([C@@H](C=C(C1)C(=O)OCC)OC(CC)CC)NC(=O)C)N.P(=O)(O)(O)O
• Target: Glycosylation
• Solubility: Soluble in DMSO (40 mg/ml); water (>40 mg/mL)

Bioactivity

• Target IC50:
  MDCK cells:1.1 μM|A/H3N2 virus:0.42 ± 0.29 µM (EC50)
• In Vivo:
  For in vivo experiments at higher doses, D-Luciferin potassium (T4139) or D-Luciferin Sodium (T19743) are recommended.
• In Vitro:
  METHODS: Preparation of D-Luciferin potassium for in vitro bioluminescence analysis: 1. Prepare 200× D-Luciferin reserve solution (30 mg/mL) with sterile water and mix gently until all dissolved. It can be used immediately after preparation or stored at -20℃. 2. Prepare 1× D-Luciferin (150 µg/mL) working solution in preheated tissue culture medium. 3. Aspirate the medium from the cultured cells. 4. Add 1× D-Luciferin solution to the cells before imaging. Incubate the cells at 37°C for a short period of time before imaging to increase signal.
• Cell Research:
  Cells are incubated in 96-well plates (5,000 cells/well) and allowed to adhere for 24 hours in 1× DMEM media containing 10% FCS. The media are replaced with fresh DMEM media containing 5% FCS with or without various concentrations of tamoxifen or OP for indicated time periods. Cell viability is tested using WST-1 cell proliferation assay.(Only for Reference)

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GS4104; Tamiflu

Compound Identifiers

PubChem CID

78000