Orotic acid monohydrate

SKU: orb1302112

Description

Orotic acid monohydrate is a key biosynthetic intermediate in the de novo pyrimidine nucleotide pathway. It is utilized in biochemical research to study nucleotide metabolism, enzyme function in vitro, and has been used in animal models to investigate related metabolic disorders.

Research Area

Metabolism Research

Key Properties

• CAS Number: 50887-69-9
• MW: 174.11
• Purity: 100.00%
• Formula: C₅H₆N₂O₅
• SMILES: O.OC(=O)C1=CC(=O)NC(=O)N1
• Target: Endogenous Metabolite
• Solubility: Ethanol:< 1 mg/mL (insoluble or slightly soluble);DMSO:2.5 mg/mL (14.36 mM);H2O:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• In Vivo:
  Male Fischer 344 rats (130-150 g) to two-thirds PH in the absence or in the presence of Orotic acid (a 300-mg tablet of Orotic acid methyl ester implanted intraperitoneally at the time of two-thirds PH). treatment with Orotic acid resulted in a near-100% inhibition of RNR induced by two-thirds PH in rat liver, as monitored by enzyme activity and protein level . Feeding 1% Orotic acid with diet decreased the phosphorylation of AMPK and increased the maturation of SREBP-1 and the expression of SREBP-responsive genes in the rat liver. Orotic acid-induced lipid accumulation was also completely inhibited by rapamycin. Mouse hepatocytes and mice were resistant to Orotic acid-induced lipogenesis because of little if any response in AMPK and downstream effectors .
• In Vitro:
  Orotic acid increases cell proliferation and decreases apoptosis in serum-starved SK-Hep1 hepatocellular carcinoma cells, which may ascribe to the inhibition of AMP-activated protein kinase (AMPK) phosphorylation and thus activation of mammalian target of rapamycin complex 1 (mTORC1) .

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, Endogenous Metabolite, EndogenousMetabolite, Orotic acid, Orotic acid Monohydrate, Orotic Acid Monohydrate, inhibit, 2,6-Dioxo-1,2,3,6-tetrahydropyrimidine-4-carboxylic acid hydrate, 2,4-dioxo-1H-pyrimidine-6-carboxylic acid hydrate, 2,4-dioxo-1H-pyrimidine-6-carboxylic acid Hydrate

Compound Identifiers

PubChem CID

16218504