ORC4L Antibody

SKU: orb1247171

Description

ORC4L Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 48kDa band observed in nuclear lysates of cell line Jurkat (calculated MW of 50.4kDa according to Human NP_002543.2 Recommended concentration: 0.3-1µg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on cell line NIH-3T3 and Mouse Heart for this particular batch.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-DVRQWATSSLSWL
• Target: ORC4L
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ORC4L, origin recognition complex, subunit 4-like (yeast), Orc4p, HSORC4, HsORC4, origin recognition complex, subunit 4 (yeast homolog)-like, Origin recognition complex, subunit 4, S. cerevisiae, homolog-like, ORC4, ORC4P, origin recognition complex subunit 4

orb1247171 (0.3 ug/ml) staining of Jurkat nuclear lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

ORC4L

UniProt

O43929

RefSeq (Protein):NP_002543.2, NP_001177810.1, NP_001177811.1