Orange I

SKU: orb1909137

Description

Orange I, also known as α-Naphthol Orange, is a synthetic sulfonated azo dye. It is utilized in research as a model compound to study microbial degradation processes, where it is noted for its resistance to breakdown, making it relevant for environmental and biochemical studies.

Key Properties

• CAS Number: 523-44-4
• MW: 350.32
• Purity: 96.48%
• Formula: C₁₆H₁₁N₂NaO₄S
• SMILES: O=S([O-])(C1=CC=C(C=C1)N=NC2=CC=C(C3=C2C=CC=C3)O)=O.[Na+]
• Target: Others
• Solubility: DMSO:35.03 mg/mL (99.99 mM)

Bioactivity

• Cell Research:
  Instructions 1. Solvent selection: Orange I is a water-soluble dye that is usually dissolved in deionized or distilled water. It has a high solubility and is usually used in a concentration of 0.01% to 0.1% (w/v). 2. Electrophoresis tracer: Orange I is commonly used as a tracer dye in gel electrophoresis and can be used to monitor the migration process of samples, especially the separation of proteins and nucleic acids. Instructions for use: Mix an appropriate amount of Orange I solution with the sample and add it to the gel. It can also be added to the electrophoresis buffer before electrophoresis. 3. pH indicator: As an acid-base indicator, Orange I can change color in the pH range of 2 to 4. It is orange at low pH and yellow at high pH. Instructions for use: It is suitable for solution pH detection, especially in acidic environments with a small pH range. 4. Staining uses: In biological experiments, Orange I is used to stain cells, tissue sections or biomolecules. For example, it is used for acid staining of cells or tissue sections. Usage: Add Orange I to the staining solution, and after staining for a certain period of time, observe the results under a microscope.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

C.I. Acid Orange 20, Japan Orange 402, Java Orange I, Nankai Acid Orange I, Orange I, sodium, Orange I, sodium salt, Tertracid Orange I, α-Naphthol Orange

Compound Identifiers

PubChem CID

135513128