ONO-5334

SKU: orb1706872

Description

ONO-5334 is a potent, selective, and orally bioavailable histone K inhibitor. It demonstrates dual activity by inhibiting SARS-CoV-2 replication and suppressing bone resorption, making it a candidate for research in osteoporosis and viral infection. Studies include in vitro antiviral assays and in vivo models of bone disease.

Research Area

Infectious Disease & Virology, Protein Biochemistry

Key Properties

• CAS Number: 868273-90-9
• MW: 438.58
• Purity: >99.99% (May vary between batches)
• Formula: C₂₁H₃₄N₄O₄S
• SMILES: C[C@@H]1CS\C(=N/NC(=O)C(=O)[C@@H](NC(=O)C2CCCCCC2)C2CCOCC2)N1C
• Target: Cysteine Protease,SARS-CoV
• Solubility: DMSO:30 mg/mL (68.4 mM)

Bioactivity

• Target IC50:
  Cathepsin K (rabbit):0.049 nM (Ki)|Cathepsin K (rat):0.85 nM (Ki)|Cathepsin K (human):0.10 nM (Ki)
• In Vivo:
  ONO-5334 (oral; 0.3-30 mg/kg; 7 consecutive days) at doses of 3 mg/kg or 30 mg/kg significantly reduced CTX (bone resorption marker) concentrations.ONO-5334 (oral; 0.12-15 mg/kg; single dose) dose-dependently reduced PTHrP-induced elevation of plasma calcium, with a significant effect at 15 mg/kg (86% reduction). It also reduced the PTHrP-induced increase in plasma CTX levels in TPTX rats by 90% at 15 mg/kg. On day 7, ONO-5334 (3 mg/kg and 30 mg/kg) reduced serum CTX concentrations by 62% and 79%, respectively.
• In Vitro:
  ONO-5334 inhibits human cathepsin S, L, and B, and porcine calpain Ι and II (Ki: 0.83 nM, 1.7 nM, 32 nM, 82 nM, and 69 nM, respectively). ONO-5334 (0.1-1 μM; 24 hours) also inhibits human osteoclast-mediated bone resorption, effectively decreasing the osteoclast-mediated release of CTX from bone slices in a dose-dependent manner.

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cysteine Protease, CysteineProtease, Cathepsin K, human cathepsin K, ONO 5334, ONO5334, ONO-5334, rat cathepsin K, rabbit cathepsin K, SARS-CoV-2, SARSCoV

Compound Identifiers

PubChem CID

11582982