Onatasertib

SKU: orb1304293

Description

Onatasertib

Research Area

Cardiovascular Research, Cell Biology, Molecular Biology, Signal Transduction

Key Properties

• CAS Number: 1228013-30-6
• MW: 397.47
• Purity: 99.93%
• Formula: C₂₁H₂₇N₅O₃
• SMILES: CO[C@H]1CC[C@@H](CC1)N1C(=O)CNc2ncc(nc12)-c1ccc(nc1)C(C)(C)O
• Target: Apoptosis,PI3K,mTOR,DNA-PK,c-Fms,FLT
• Solubility: DMSO:70 mg/mL (176.11 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (5.03 mM);Ethanol:73 mg/mL (183.66 mM);H2O:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  mTOR:16 nM|PI3Kα:4 μM|DNA-PK:0.84 μM
• In Vivo:
  In numerous cell lines, CC-223 effectively inhibits markers of both mTORC1 (S6RP and 4EBP1) and mTORC2 [AKT(S473)], with IC50 Values for pAKT(S473) ranging from 11-150 nM, pS6RP from 27-184 nM, and p4EBP1 from 120-1050 nM. Additionally, CC-223 suppresse the growth of various cancer cell lines and induces apoptosis.
• In Vitro:
  In mice with PC-3 tumor xenografts, CC-223 (25 mg/kg, p.o.) inhibite the activity of both mTORC1 and mTORC2. Additionally, CC-223 (25 mg/kg, p.o.) suppressed tumor growth across several models, including those for prostate cancer, glioblastoma, breast cancer, lung cancer, and colorectal cancer, achieving Inhibition rates between 47%-95%.
• Cell Research:
  Compound is spotted via an acoustic dispenser (EDC ATS-100) into an empty 384-well plate. Cells are diluted to desired densities and added directly to the compound-spotted plates. Cells are allowed to grow for 72 hours. Viability is assessed via Cell Titer-Glo. All data are normalized and represented as a percentage o the DMSO-treated cells. Results are then expressed as GI50 and/or IC50 values.(Only for Reference)

Storage & Handling

• Storage: store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

mTOR, inhibit, Onatasertib, FLT4, Inhibitor, Mammalian target of Rapamycin, DNAPK, DNA-PK, CC223, CC-223, CC 223, Apoptosis, ATG 008, ATG008, ATG-008, cFms, c-Fms, PI3K-α

Compound Identifiers

PubChem CID

58298316