Oligomycin A

SKU: orb1300870

Description

Oligomycin A is a potent and specific inhibitor of mitochondrial ATP synthase, effectively blocking oxidative phosphorylation. It is widely used in both in vitro and in vivo research to study cellular bioenergetics, metabolic pathways, and the role of mitochondrial function in various disease models.

Research Area

Cell Biology

Key Properties

• CAS Number: 579-13-5
• MW: 791.1
• Purity: >98%
• Formula: C₄₅H₇₄O₁₁
• SMILES: [H][C@]12CC[C@@H](CC)\C=C\C=C\C[C@@H](C)[C@H](O)[C@@](C)(O)C(=O)[C@@H](C)[C@H](O)[C@@H](C)C(=O)[C@@H](C)[C@H](O)[C@@H](C)\C=C\C(=O)O[C@]([H])([C@@H]1C)[C@H](C)[C@@]1(CC[C@H](C)[C@H](C[C@@H](C)O)O1)O2
• Target: ATP synthase
• Solubility: Soluble in DMSO (300 mg/ml); or Ethanol (200 mg/ml)

Bioactivity

• Target IC50:
  HCT116 cells:0.9 μM|K562 cells:0.2 μM|F0F1-ATPase:1 μM (Ki)|SH-SY5Y cells:29 μM|HCT116 cells:1 μM
• In Vivo:
  METHODS: To assay anti-inflammatory activity, Oligomycin A (0.25 mg/kg) was injected intraperitoneally into C57BL6 mice in a psoriasis model once daily for five days. RESULTS: Oligomycin A reduced ear thickness, keratinocyte proliferation and immune cell infiltration.
• In Vitro:
  METHODS: Human melanoma cells WM3734 were treated with Oligomycin A (0.01-1 µg/mL) for 24 h, and lactate production was measured in culture supernatants. RESULTS: Oligomycin A caused an increase in lactate production in WM3734 cells. METHODS: Human lung cancer cells H1299 were treated with Oligomycin A (0.1-1000 ng/mL) for 100 min, and cellular respiration was measured using Oxygen Biosensor System plates. RESULTS: At 100 and 1000 ng/mL, Oligomycin A completely inhibited cellular respiration within approximately one hour.
• Cell Research:
  ATP and Oligomycin Dose-Response Growth Measurement. Cellular ATP changes are measured by CellTiter-Glo reagent. To measure oligomycin dose-response curves, the cells are plated in 96-well plates at about 400–500 cells/well in 100 μl of culture, dosed the next day, and grown for 4 additional days followed by assaying with Cell-Titer-Glo reagent. The dose-response curves are plotted with nonlinear regression analysis of GraphPad Prism.(Only for Reference)

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

Fungal, Inhibitor, MCH32, MCH-32, MCH 32, Oligomycin A, inhibit, ATP synthase, ATP Synthase, Antibiotic

Compound Identifiers

PubChem CID

52947716