Oil Red O

SKU: orb1297708

Description

Oil Red O is a lysochrome diazo dye used to stain neutral lipids and lipoproteins in frozen tissue sections. It is widely applied in histopathology and lipid metabolism research, including studies of atherosclerosis, fatty liver disease, and adipogenesis in both in vitro and in vivo models.

Key Properties

• CAS Number: 1320-06-5
• MW: 408.49
• Purity: ≥98% (May vary between batches)
• Formula: C₂₆H₂₄N₄O
• SMILES: Cc1cccc(\N=N\c2ccc(\N=N\c3c(O)ccc4ccccc34)c(C)c2C)c1C
• Target: Others
• Solubility: DMSO:1.25 mg/mL (3.06 mM)

Bioactivity

• Cell Research:
  Instructions: a. Reagent preparation: Prepare Oil Red O stock solution, dissolve 0.5g Oil Red O in 100mL 98% isopropanol; for working solution, mix 24mL of stock solution with 16mL of distilled water, filter and use. Also prepare Mayer's hematoxylin stain and 1% lithium carbonate solution. b. Operation steps: 1. Cut frozen tissue sections with a thickness of 10µm and dry them at room temperature for 10 minutes; 2. Use a liquid antifouling pen to draw along the area around each section; 3. Add 1mL of Oil Red O working solution, incubate at room temperature for 10 minutes (for heart and liver sections, incubation for 5 minutes is recommended; for muscle sections, incubation for 10 minutes), and wash with water; 4. Stain with Mayer's hematoxylin stain for 2 minutes and wash with water; 5. Blue in lithium carbonate solution for 30 seconds to 1 minute, wash with water, ((d) (e) optional, this step can show cell nuclei and tissue morphology, but will interfere with lipid quantification); 6. Seal with aqueous mounting medium; 7. Observe and photograph under an optical microscope. Stained sections should be photographed within 24 hours to avoid precipitation of Oil Red O dye.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, hepatic steatosis, inhibit, lipid, ORO, Oil Red O, metabolic diseases

CAS: 1320-06-5

Compound Identifiers

PubChem CID

3033868