NVP 231

SKU: orb1225200

Description

NVP-231 is a potent, specific, and reversible CerK inhibitor(IC50=12±2 nM) that competitively inhibits binding of ceramide to CerK.

Key Properties

• CAS Number: 362003-83-6
• MW: 431.55
• Purity: >98% (HPLC)
• Formula: C₂₅H₂₅N₃O₂S
• SMILES: O=C(C1(C2)C[C@H]3C[C@@H]2C[C@H](C3)C1)NC4=CC=C5N=C(NC(C6=CC=CC=C6)=O)SC5=C4
• Target: ERK
• Solubility: Soluble in DMSO

Bioactivity

• In Vitro:
  Cell Viability Assay Cell line: MCF-7 cells; NCI-H358 cells. Concentration: 0 nM, 10 nM, 100 nM, 300 nM, 500 nM, 1000 nM. Incubation time: 48 hours. Result: Reduced MCF-7 cells and NCI-H358 cells in a concentration manner. Apoptosis Analysis Cell line: MCF-7 cells; NCI-H358 cells. Concentration: 1000 nM. Incubation time: 24-72 hours. Result: Increases caspase-3 and caspase-9 cleavage in MCF-7 and NCI-H358 cells. Western blot analysis. Cell line: MCF-7 cells; NCI-H358 cells. Concentration: 0 nM, 100 nM, 300 nM, 500 nM. Incubation time: 24 hours. Result: Decreased p-cyclin B1, p-CDK1 as a concentration manner.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NVP231 | NVP 231 | NVP-231

Quick Database Links

Gene Symbol

ERK