Nutlin-3b

SKU: orb1300615

Description

Nutlin-3b

Research Area

Cell Biology, Protein Biochemistry

Key Properties

• CAS Number: 675576-97-3
• MW: 581.49
• Purity: 98.83%
• Formula: C₃₀H₃₀Cl₂N₄O₄
• SMILES: COc1ccc(C2=N[C@@H]([C@@H](N2C(=O)N2CCNC(=O)C2)c2ccc(Cl)cc2)c2ccc(Cl)cc2)c(OC(C)C)c1
• Target: MDM-2/p53,E1/E2/E3 Enzyme,Mdm2
• Solubility: DMSO:93 mg/mL (159.93 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.3 mg/mL (5.68 mM);Ethanol:93 mg/mL (159.93 mM);H2O:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  MDM2:13.6 μM
• In Vitro:
  Nutlin-3b is useful as a negative control s for non-MDM2-related cellular activities. Nutlin-3a induces the expression of MDM2 and p21 (but not p53) only in cells with wild-type p53. Nutlin-3b has no effect regardless of the p53 status of the cells. Only the active enantiomer Nutlin-3a shows a potent antiproliferative activity and clear separation of potency between the cells harboring wild-type p53 and those harboring mutant p53. The potency of Nutlin-3b is much lower in the wild-type p53 cells and nearly identical to the potency of Nutlin-3a against the mutant p53 cells. After 48 hours of exposure to the Nutlin-3a, 45% of the cell population became TUNEL positive, but cells treated with the Nutlin-3b are indistinguishable from the untreated controls.
• Cell Research:
  MTT assay(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

E2Enzyme, E3 Enzyme, E1/E2/E3 Enzyme, E1 activating enzyme, E1 Enzyme, E2 conjugating enzyme, E2 Enzyme, E1Enzyme, (+)-Nutlin-3, Inhibitor, inhibit, Nutlin 3b, Nutlin-3, Nutlin3b, Nutlin-3b, E3Enzyme, E3 ligating enzyme, MDM-2/p53, Mdm2, Ubiquitin activating enzyme, Ubiquitin ligase, Ubiquitin conjugating enzyme

Compound Identifiers

PubChem CID

16755649