NU1025

SKU: orb1300352

Description

NU1025 (NSC-696807) is a small molecule inhibitor of PARP with an IC50 value of 400 nM. It is a research tool used in vitro and in vivo to study DNA repair mechanisms, synthetic lethality, and oncology, particularly in BRCA-deficient cancer models.

Research Area

Epigenetics & Chromatin, Molecular Biology

Key Properties

• CAS Number: 90417-38-2
• MW: 176.17
• Purity: 99.86% (May vary between batches)
• Formula: C₉H₈N₂O₂
• SMILES: Cc1nc2c(O)cccc2c(=O)[nH]1
• Target: PARP
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (11.35 mM);DMSO:40 mg/mL (227.05 mM);1eq. NaOH:17.6 mg/mL (99.9 mM)

Bioactivity

• Target IC50:
  PARP:400 nM
• In Vivo:
  Treatment with NU1025 (1 and 3 mg/kg) reduces the infarction to 25% and 45% versus vehicle treated rats, respectively. NU1025 (1 and 3 mg/kg) treatment significantly reduces edema volume. NU1025 also produces significant improvement in neurological deficits.
• In Vitro:
  NU1025 (0.2 mM) treatment attenuates Water2 induced cytotoxicity. NU1025 per se does not have any effect on cell viability. NU1025 pretreatment significantly increases cell viability (82.59 4.67%) in SIN-1 (0.8 mM) exposed cells. NU1025 has no detectable effect on the proliferation of D54 and U251 cells. Treatment with NU1025 markedly inhibits the enhanced activation of PARP-1 induced by TPT and RT treatment. No DNA strand breakage is detected following exposure to 200 μM NU1025 alone.
• Cell Research:
  Cells are seeded in 96-well plates at a density of 2,500 cells/well and treated with the indicated doses of NU1025. Adherent cells are irradiated in medium with 250 kVp X-rays (dose rate 0.5 Gy/min). Untreated cells are used as a control. Following an up to 5 day incubation, cell proliferation is assessed by MTT assay.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA, cytotoxicity, anti-cancer, Inhibitor, NSC696807, NSC-696807, NU1025, NU-1025, NU 1025, Neuroprotective, NAD, PAR, PARP, NSC 696807, inhibit, repair, poly ADP ribose polymerase

Compound Identifiers

PubChem CID

135398517